| Cat.No.: | EAb-0610 |
| Product Name: | Histone H3 K4me3/pT6 Polyclonal Antibody |
| Product Overview: | Anti-Histone H3 [Trimethyl Lys4, p Thr6] (RABBIT) Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2. |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications: | Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot |
| Recommended Dilutions/Conditions: |
ChIP: 2-5µg/million cells; IF Microscopy: 1:50; Western Blot: 1:500; Immunohistochemistry: 1:50; Dot Blot: 0.5µg/mL Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.01% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | UniprotKB - Q71DI3; NCBI - NP_001005464; GeneID - 126961 |
| Alternative Name: | rabbit anti-Histone H3 trimethyl Lys4 pT6 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me3/pT6 |
| Scientific Background: | Phosphorylation at T6 of methylated H3K4 prevents LSD1 from demethylating histone H3. Androgen receptor activated gene expression depends upon removal of methyl groups from H3K4, in cooperation with the Jumonji protein JMJD2C. However, when T6 is phosphorylated, there is a physical obstruction in the way of demethylation, and thus gene expression is repressed. The PHD finger of H3K4 seems to be an effector of histone modification, which can cause dysfunction in cellular fate regulation. Interestingly, the abundance of phosphorylation of this modified histone is a probable biomarker for the detection and the prognosis of certain cancers. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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