| Cat.No.: | EAb-0548 |
| Product Name: | Histone H3K9me3 Polyclonal Antibody |
| Product Overview: | Anti-Histone H3 [Trimethyl Lys9] (RABBIT) Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 9 of human Histone H3.2. |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications: | Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence |
| Recommended Dilutions/Conditions: |
ChIP: 2-5µg/million cells; IF Microscopy: 1:1000; Western Blot: 1:5000; Immunohistochemistry: 1:1000; Dot Blot: 0.5µg/mL Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.01% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | UniprotKB - Q71DI3; NCBI - NP_001005464; GeneID - 126961 |
| Alternative Name: | rabbit anti-Histone H3 trimethyl Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me3 |
| Scientific Background: | Transcriptional silencing of specific genes and repetitive elements is known to be regulated partially through methylation of histone H3 at lysine 9, a hallmark of constitutive heterochromatin. In placenta, OCT2 expression and its ultimate function as a cation transporter is linked with the abundance of H3K9me3; when this methylation of histone H3 occurs in excess, mRNA for OCT2 decreases. Centromeres also are distinctly affected by the H3K9me3 modification, in that it co-localizes with several centromeric proteins, and affects the DNA methylation, particularly at the periphery of centromeric DNA. Gene expression is silenced when there is coordination between the H3K9me3 and H3K4modifications. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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