| Cat.No.: | EAb-0530 |
| Product Name: | di-methyl-Histone H3 (Lys4) Monoclonal Antibody ChIP Grade |
| Description: | The mouse-derived antibody is purified with protein G-conjugated agarose followed by di-methylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with lysine di-methylation at Lys4. |
| Antibody Type: | Monoclonal |
| Immunogen: | Synthetic di-methyl peptide corresponding to residues surrounding Lys4 of human histone H3 |
| Host: | Mouse |
| Isotype: | IgG |
| Specificity: | H3 with lysine di-methylation at Lys4 |
| Purification: | Antibodies are purified by protein G-conjugated agarose followed by di-methylated histone H3 (Lys4) peptide affinity chromatography. |
| Appearance: | Liquid |
| Applications: | ChIP, IP |
| Recommended Dilutions/Conditions: |
IP: 1:25; ChIP: 1:25 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Eukaryotes |
| Storage: | -20°C |
| Storage Buffer: | PBS with 50% glycerol and 0.01% sodium azide. |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Scientific Background: | The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine methylation inmultiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of activeand in active regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore,enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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