| Cat.No.: | EAb-0488 |
| Product Name: | Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Polyclonal Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164] |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | KDM4A |
| Purification: | Immunogen affinity purified |
| Appearance: | Liquid |
| Formulation: | PBS and 30% Glycerol |
| Applications: | WB, Simple Western, ICC/IF, IHC, IHC-P |
| Recommended Dilutions/Conditions: |
Western Blot 1:25000; Simple Western 1:500; Immunocytochemistry/Immunofluorescence 1:100; Immunohistochemistry 1:50; Immunohistochemistry-Paraffin 1:50 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Mouse |
| Storage: | -20°C |
| Storage Buffer: | 0.05% Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | EC 1.14.11; EC 1.14.11.-; JHDM3A; JHDM3AJMJD2; JmjC domain-containing histone demethylation protein 3A; JMJD2; JMJD2A; JMJD2Alysine-specific demethylase 4A; jumonji domain containing 2; jumonji domain containing 2A; Jumonji domain-containing protein 2A; KDM4A; KIAA0677jumonji C domain-containing histone demethylase 3A; Lysine (K)specific Demethylase 4A; Lysine (K)-specific Demethylase 4A |
| Scientific Background: | Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| EAb-0072 | JMJD6 Polyclonal Antibody | Inquiry |
| EAb-0074 | JMJD2c Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| JMJD | JMJD1A | JMJD1B | JMJD1C |
| JMJD2 | JMJD2A | JMJD2B | JMJD2C |
| JMJD2D | JMJD2E | JMJD2F | JMJD3 |
| JMJD4 | JMJD5 | JMJD6 | KDM1 |
| KDM1A | KDM1B | KDM2 | KDM2A More > |
| KDM2B | KDM3A | KDM3B | KDM4 |
| KDM4A | KDM4B | KDM4C | KDM4D |
| KDM4E | KDM5 | KDM5A | KDM5B |
| KDM5C | KDM5D | KDM6A | KDM6B |
| KDM6C | KDM7 | KDM7A | KDM7B |
| KDM8 | |||
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
