| Cat.No.: | EAb-0427 |
| Product Name: | DNMT1 Polyclonal Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Contained within amino acids 1-125 of the N-terminus of human Dnmt1. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | DNMT1 |
| Purification: | Unpurified |
| Appearance: | Liquid |
| Formulation: | Whole antisera |
| Applications: | WB, Simple Western, ICC/IF, IHC, IHC-P, IP, KD, KO |
| Recommended Dilutions/Conditions: |
Western Blot; Simple Western 1:100; Immunocytochemistry/Immunofluorescence 1:50 - 1:200; Immunohistochemistry 1:100; Immunohistochemistry-Paraffin 1:100; Immunoprecipitation 1:10 - 1:500; Knockdown Validated; Knockout Validated Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Mouse |
| Storage: | -20°C |
| Storage Buffer: | No Preservative |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P26358 |
| Alternative Name: | AIM; CXXC9; CXXC9CXXC finger protein 9; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA MTase HsaI; DNMT; DNMT1; EC 2.1.1.37; FLJ16293; m.HsaI; MCMT; MCMTMGC104992 |
| Scientific Background: | Member of C5-methyltransferase family and the most abundant DNA methyltransferase in mammalian cells, Dnmt1 (DNA methyltransferase 1) is a key methylation enzyme implicated in maintaining methylation during DNA replication/repair which has capability of methylating CpG residues with preference to hemimethylated DNA and de novo methylation during somatic cell development/differentiation. Dnmt1 interacts with CSNK1D, HDAC1, PCNA, MBD2, MBD3, DNMT3A, DNMT3B, PRC2/EED-EZH2 complex, UBC9, BAZ2A/TIP5, UHRF1, USP7 etc., and forms complexes with DMAP1, HDAC2, E2F1, BB1, HDAC1 and SUV39H1. In cell division, Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in newly synthesized strand, that is essential for epigenetic inheritance. Moreover, it associates with chromatin during G2 as well as M phases to maintain DNA methylation independently of replication, and is reduced to non detectable levels at the G0 phase with dramatic induction upon entry into S-phase. Dnmt1 mediates transcriptional repression by direct binding to HDAC2 and in association with DNMT3B/via the recruitment of CTCFL/BORIS, it involves in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 as well as H3K9. Dnmt1 is responsible for maintaining methylation patterns established in development and defects in DNMT1 are the cause of hereditary sensory neuropathy type 1E (HSN1E). |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0008 | Vinorelbine Tartrate | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0027 | Recombinant Human DNMT3A 293 Cell Lysate | Inquiry |
| EL-0028 | Recombinant Human DNMT3B 293 Cell Lysate | Inquiry |
| EL-0029 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| EL-0030 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| DNA alkyltransferase | DNAJC2 | DNAS1L1 | DNASE1L3 |
| DNMT | DNMT1 | DNMT2 | DNMT3A |
| DNMT3B | DNMT3L | DNMT4 | |
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