| Cat.No.: | EAb-0287 |
| Product Name: | DNA Methyltransferase 3a Monoclonal Antibody (Clone 64B814.1) |
| Product Overview: | Monoclonal antibody to detect DNMT3a in Human and Mouse samples |
| Antibody Type: | Monoclonal |
| Clone Designation: | 64B814.1 |
| Immunogen: | Recombinant mouse His-tagged DNMT3a expressed in bacteria |
| Host: | Mouse |
| Isotype: | IgG1κ |
| Antibody Target: | DNMT3A |
| Purification: | Protein G purified |
| Appearance: | Liquid |
| Formulation: | 100 µg protein G-purified IgG in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide |
| Applications: | Western blot, Immunofluorescence, Immunocytochemistry |
| Recommended Dilutions/Conditions: |
Western blot: 1-3 µg/ml, IF/ICC: 5 µg/ml. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western blot: Transfected 293 cell lysate, untransfected 293 cell lysate |
| Species Reactivity: | Human, mouse |
| Shipping: | Wet ice |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | O88508 |
| Alternative Name: | DNMT3a |
| Scientific Background: | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0008 | Vinorelbine Tartrate | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0027 | Recombinant Human DNMT3A 293 Cell Lysate | Inquiry |
| EL-0028 | Recombinant Human DNMT3B 293 Cell Lysate | Inquiry |
| EL-0029 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| EL-0030 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| DNA alkyltransferase | DNAJC2 | DNAS1L1 | DNASE1L3 |
| DNMT | DNMT1 | DNMT2 | DNMT3A |
| DNMT3B | DNMT3L | DNMT4 | |
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