| Cat.No.: | EAb-0246 |
| Product Name: | Histone H1 Recombinant Antibody (r1415-1) |
| Antibody Type: | Recombinant |
| Immunogen: | Nuclei of human leukemia biopsy cells. |
| Host: | Mouse |
| Isotype: | lgG2aκ |
| Purification: | Protein G affinity purified. |
| Appearance: | Liquid |
| Formulation: | In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide. |
| Applications: | Flow Cytometry, IF, IHC (PS) |
| Recommended Dilutions/Conditions: |
Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL)Immunofluorescence (0.5-1 µg/mL)Immunohistochemistry (formalin-fixed paraffin sections) (0.5-1 µg/mL for 30 min at RT)Western blot (0.5-1 µg/mL). Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Mouse, Rat |
| Shipping: | Blue Ice |
| Storage: | +4°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P07305 |
| Scientific Background: | Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
