| Cat.No.: | EAb-0127 |
| Product Name: | Histone H3K27me3/pS28 Polyclonal Antibody |
| Product Overview: | Polyclonal Antibody to detect H3K27me3S28p in human samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | KLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | H3K27me3S28p |
| Purification: | Whole antiserum from rabbit |
| Appearance: | Colorless liquid |
| Formulation: | In PBS with 0.05% (W/V) sodium azide. |
| Applications: | Western blot, IF, ELISA, ChIP, Dot blot. |
| Recommended Dilutions/Conditions: |
IF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western blot: HeLa cells, IF: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides. |
| Species Reactivity: | Human |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P68431 |
| Alternative Name: | Histone H3 |
| Scientific Background: | Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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