| Cat.No.: | EAb-0113 |
| Product Name: | Histone H3K9me3 Polyclonal Antibody |
| Product Overview: | Polyclonal Antibody to detect H3K9me3 in human samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 9 (H3K9me3), using a KLH-conjugated synthetic peptide. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | H3K9me3 |
| Purification: | Affinity Purified |
| Appearance: | Colorless liquid |
| Formulation: | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
| Applications: | Western blot, IF, ELISA, Dot Blot, ChIP. |
| Recommended Dilutions/Conditions: |
WB: 1:1000, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IF: 1:500, ELISA: 1:1000. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western blot: HeLa cells, IF: NIH3T3 cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides. |
| Species Reactivity: | Human |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P68431 |
| Alternative Name: | Histone 3 |
| Scientific Background: | Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K9 is associated with satellite repeat regions and ZNF repeat genes. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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