| Cat.No.: | EAb-0055 |
| Product Name: | Phospho-DDX3X (Thr322) Polyclonal Antibody |
| Product Overview: | Polyclonal antibody to detect endogenous levels of DDX3X (pT322) in human. |
| Antibody Type: | Polyclonal |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DDX3X |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | DDX3X |
| Purification: | Affinity purified |
| Appearance: | Colorless liquid |
| Formulation: | In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide |
| Applications: | WB, IH, IF/IC |
| Recommended Dilutions/Conditions: |
WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | WB: HeLa cell lysates; IHC: human brain tissue; IFC HeLa cells |
| Species Reactivity: | Human |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | O00571 |
| Alternative Name: | DBX, DDX3, ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal; DEAD box X isoform, Helicase-like protein 2, HLP2 |
| Scientific Background: | Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs. Involved in transcriptional regulation. Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner. Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0055 | Phospho-DDX3X (Thr322) Polyclonal Antibody | Inquiry |
| EAb-0306 | DDX43 Polyclonal Antibody | Inquiry |
| EAb-0307 | DDB2 Polyclonal Antibody | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0057 | Recombinant Human DDX20 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0178 | Human RIG-I/DDX58 peptide | Inquiry |
| Related Gene / Proteins | |||
| DDB2 | Ddit3 | DDX10 | DDX18 |
| DDX20 | DDX28 | DDX3X | DDX41 |
| DDX42 | DDX43 | DDX49 | DDX50 |
| DDX58 | |||
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