USP1 Polyclonal Antibody

Cat.No.:	EAb-0009
Product Name:	USP1 Polyclonal Antibody
Product Overview:	Polyclonal antibody to detect USP1 (a DUB enzyme) in human, mouse and rat samples.
Antibody Type:	Polyclonal
Immunogen:	Fusion protein of human USP1
Host:	Rabbit
Isotype:	IgG
Antibody Target:	USP1
Purification:	Affinity purified
Appearance:	Colorless liquid
Formulation:	100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Applications:	Western blot, Immunohistochemistry
Recommended Dilutions/Conditions:	Western blot: 1:200 – 1:500 dilution, IHC: 1:50 - 1:100 dilution. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user.
Positive Control:	Western Blot: HEK-293 & HeLa cell lysates
Species Reactivity:	Human, mouse, rat
Shipping:	Gel Pack
Storage:	-20°C
Warning:	For Research Use Only! Not For Use in Humans.

Accession:	O94782
Alternative Name:	USP1, UBP
Scientific Background:	Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitinproteosomal mechanisms. USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNAmediated translation synthesis (TLS) during DNA repair. USP1 interaction with UAF1, a WD40 repeat containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.