| Cat.No.: | EL-0410 |
| Product Name: | C2C12 nuclear extract (Undifferentiated) |
| Description: | C2C12 nuclear extract was prepared from subconfluent undifferentiated cell cultures of the mouse myoblast C2C12 cell line. Under certain conditions, such as androgen stimulation, these fibroblast cells can differentiate to a skeletal muscle phenotype, forming contractile myotubes and expressing characteristic skeletal muscle proteins. When treated with bone morphogenic protein 2 (BMP-2), these cells shift differentiation pathways from myoblastic to osteoblastic and are useful in studies examining BMP-mediated Smad signaling. Due to their differentiative potential, these cells are commonly used as cell models to study skeletal muscle and bone differentiation. In addition, these cells express TGFβ receptors and are also commonly used in studies related to the TGF-β signaling pathway. |
| Species: | Murine |
| Source: | Murine Muscle Myoblast |
| Components: | 200 µg of C2C12 (Undifferentiated) nuclear extract at 5.7 µg/µl. |
| Extract Composition: | C2C12 nuclear extract (Undifferentiated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). |
| Application Notes: | C2C12 nuclear extract is specifically recommended for analysis of 1) myoblast differentiation, 2) osteoblast differentiation, and 3) TGFβ signaling cascades. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
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