| Cat.No.: | EL-0404 |
| Product Name: | COS-7 nuclear extract (IFNγ treated) |
| Description: | COS-7 nuclear extract (IFNγ treated) was prepared from subconfluent undifferentiated cell cultures of the Simian kidney fibroblast COS-7 cell line. The COS-7 cell line was derived from immortalizing the African green monkey CV-1 cell line by transformation with an origin-defective mutant of the SV40 viral genome that is replication-deficient but produces wild-type large T antigen. The large T antigen supports replication of and lytic growth of the SV40 virus as well as replication of SV40 promoter-containing plasmids. In addition to supporting propagation of the Simian SV40 virus, the COS-7 cell line is most commonly used in research related to replication and in transfection studies for the production of recombinant proteins. Treatment of COS-7 cells with IFNγ promotes apoptosis, causes alterations in viral replication efficiency, and induces the JAK/STAT signaling pathway. |
| Species: | Monkey |
| Source: | Monkey Kidney, SV40 transformed |
| Components: | 2 x 100 µg of COS-7 nuclear extract (IFNγ treated) at 2.5 µg/µl. |
| Extract Composition: | COS-7 nuclear extract is supplied in Dilution Buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors). Cells were treated with 1000 U/ml of IFNγ for 15 minutes immediately prior to harvesting. |
| Application Notes: | COS-7 nuclear extract (IFNγ treated) is specifically recommended for 1) analysis of replication, 2) for transfection studies, and 3) studies of apoptosis. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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