| Cat.No.: | EL-0387 |
| Product Name: | HL-60 nuclear extract |
| Description: | HL-60 nuclear extract was prepared from a cell culture of the human promyelocytic leukemia HL-60 cell line. The HL-60 cell line was originally derived from tumor tissue obtained from a 36 year old female patient with acute promyelocytic leukemia. HL-60 cells are primarily neutrophilic myelocyte precursors in morphology and can be induced to differentiate into granulocytic, monocytic, macrophage-like and eosinophil phenotypes depending on the compound treatment. These cells require insulin and transferrin for growth. In research, HL-60 cells are most commonly used to study myeloid differentiation, hematopoiesis, and acute promyelocytic leukemia. Historically, this cell line was used as a model system to study the effect of DNA topoisomerase IIα and IIβ on differentiation and apoptosis of cells. |
| Species: | Human |
| Source: | Human myeloblastic leukemia (leukocytes) |
| Components: | 200 µg of HL-60 nuclear extract at 7.72 µg/µl. |
| Extract Composition: | HL-60 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). |
| Application Notes: | HL-60 nuclear extract is recommended for use in studies related to 1) myeloid differentiation, 2) hematopoiesis, and 3) acute promyelocytic leukemia. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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