| Cat.No.: | EL-0328 |
| Product Name: | HeLa acid extract (Sodium butyrate treated) |
| Description: | HeLa acid extract (Sodium butyrate treated) was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line that was treated with sodium butyrate. Acid extraction is performed under conditions that are optimized for solubility of histone proteins. Sodium butyrate is a compound that has profound effects on the morphology, growth rate and gene expression of mammalian cultured cells. The primary mechanism of action of sodium butyrate is the inhibition of the activity of histone deacetylases (HDACs) that results in the extensive, although reversible, acetylation of histones. In HeLa cells, butyrate-mediated histone hyperacetylation leads to inhibition of DNA synthesis and arrest in G1, induction of differentiation, and alterations in gene expression. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 100 µg HeLa acid extract (Sodium butyrate treated) at 0.5 mg/ml. |
| Extract Composition: | HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF). Cells were cultured in medium containing 5 mM sodium butyrate for 12 hours immediately prior to harvesting. |
| Application Notes: | Acid extracts are commonly utilized for isolation and purification of histones. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
