Recombinant Human DNMT3B 293 Cell Lysate


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  • Gene Information
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Cat.No.:  EL-0028
Product Name:  Recombinant Human DNMT3B 293 Cell Lysate
Description:  Antigen standard for DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Species:  Human
Source:  HEK 293 Cells
Components:  This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Storage:  Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  1789
Official Symbol:  DNMT3B
Official Full Name:  DNA (cytosine-5-)-methyltransferase 3 beta
Synonyms:  DNMT3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; ICF; ICF1; M.HsaIIIB;
mRNA Refseq:  NM_001207055
Protein Refseq:  NP_001193984
MIM:  602900
UniProt ID:  Q9UBC3
Chromosome Location:  20q11.2
Function:  DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; metal ion binding; protein binding; transcription corepressor activity; transcription corepressor activity; transferase activity; unmethylated CpG binding;

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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