Cat.No.: | EKIT-0585 |
Product Name: | Express DNA methylation kit |
Size: | 200 tests; 50 tests |
Product Overview: | Fastest method of complete bisulfite conversion of DNA for methylation treatment |
Description: | The Express DNA methylation kit can be used for rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis. Simply add the Express Conversion Reagent for the reaction to occur. With the addition of heat, DNA denaturation is facilitated. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc. |
Background: | Cytosine methylation is a naturally occurring base modification, in both prokaryotic and eukaryotic organisms. It involves the addition of a methyl group to the fifth carbon position of the cytosine pyrimidine ring via a methyltransferase enzyme. It has been demonstrated that aberrant DNA methylation is a widespread phenomenon in cancer and may be among the earliest changes to occur during oncogenesis. DNA methylation has also been shown to play a central role in gene imprinting, embryonic development, X-chromosome gene silencing, and cell cycle regulation. |
Kit Components: | Express conversion reagent, Methylation binding buffer, Methylation wash buffer concentrate, Methylation elution buffer , IC column, IC collection tube, L-Desulphonation reagent |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Research Kits | ||
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USA
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