| Cat.No.: | EKIT-0583 |
| Product Name: | Blood and tissue DNA methylation kit |
| Size: | 200 tests; 50 tests |
| Product Overview: | Complete bisulfite conversion from blood and tissue samples |
| Description: | The Blood and tissue DNA methylation kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50pg DNA. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc. |
| Background: | Cytosine methylation is a naturally occurring base modification, in both prokaryotic and eukaryotic organisms. It involves the addition of a methyl group to the fifth carbon position of the cytosine pyrimidine ring via a methyltransferase enzyme. It has been demonstrated that aberrant DNA methylation is a widespread phenomenon in cancer and may be among the earliest changes to occur during oncogenesis. DNA methylation has also been shown to play a central role in gene imprinting, embryonic development, X-chromosome gene silencing, and cell cycle regulation. |
| Kit Components: | Proteinase K and storage buffer, Methylation digestion buffer (2x), BT conversion reagent, Methylation dilution buffer, Methylation-solubilization buffer, Methylation reaction buffer, Methylation binding buffer, Methylation wash buffer concentrate, M-desulphonation buffer, Methylation elution buffer, IC columns, Collection tubes |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
