| Cat.No.: | EKIT-0554 |
| Product Name: | FTO Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. |
| Background: | The methylation of internal adenosines at the N6 position (m6A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer’s disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m6A demethylase identified. |
| Applications: | For studying enzyme kinetics and HTS applications. |
| Kit Components: | The FTO Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate precoated with methylated FTO substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified FTO for 96 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0554 | FTO Chemiluminescent Assay Kit | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-1797 | FTO, His-Tag (Sf9 derived) | Inquiry |
| PE-1800 | FTO , His-Tag (E. coli derived) | Inquiry |
| PE-2380 | Recombinant Human FTSJ3 protein | Inquiry |
| ◆ Antibodies | ||
| EAb-1986 | FTO Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| FTO | FTSJ2 | FTSJ3 | FTSJD2 |
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