| Cat.No.: | EKIT-0553 |
| Product Name: | LSD1 (KDM1A) Fluorescent Assay Kit |
| Size: | 96 reactions |
| Description: | The LSD1 Assay Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. The key to the LSD1 Assay Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (λex=530±13 nm, λem=590±18 nm) is measured using a plate reader. |
| Background: | LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development. |
| Applications: | For studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Kit Components: | The LSD1 Assay Kit is provided in a convenient 96-well format, with purified LSD1, AmplexRed reagent, peroxidase, and LSD1 assay buffer for 100 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0062 | Histone Demethylase LSD1 Activity/Inhibition Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | LSD1 | LSD2 |
| LSM2 | LSM3 | LSM5 | LSM6 |
| LSP1 | |||
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