Cat.No.: | EKIT-0546 |
Product Name: | PHF8 (KDM7B) Homogeneous Assay Kit |
Size: | 384 reactions |
Description: | The PHF8 Homogeneous Assay Kit is designed to measure the activity of PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. |
Applications: | For studying enzyme kinetics and HTS applications. |
Kit Components: | The PHF8 (KDM7B) Homogeneous Assay Kit comes in a convenient AlphaLISA format with primary antibody 17-4, FLAG-tagged PHF8, and assay buffers for 384 reactions. |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Cell Lines | ||
CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
◆ Antibodies | ||
EAb-0056 | PHF8 Polyclonal Antibody | Inquiry |
EAb-0058 | PHC2 Polyclonal Antibody | Inquiry |
EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
Related Gene / Proteins | |||
KDM1 | KDM1A | KDM1B | KDM2 |
KDM2A | KDM2B | KDM3A | KDM3B |
KDM4 | KDM4A | KDM4B | KDM4C |
KDM4D | KDM4E | KDM5 | KDM5A |
KDM5B | KDM5C | KDM5D | KDM6A More > |
KDM6B | KDM6C | KDM7 | KDM7A |
KDM7B | KDM8 | PHB | PHC1 |
PHC2 | PHD | PHD1 | PHD2 |
PHD3 | PHF1 | PHF10 | PHF13 |
PHF17 | PHF2 | PHF20 | PHF20B |
PHF21A | PHF21B | PHF6 | PHF8 |
PHIP | PHPT1 |
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