SMARCA4/BRG1 Inhibitor Screening Assay Kit


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Cat.No.:  EKIT-0533
Product Name:  SMARCA4/BRG1 Inhibitor Screening Assay Kit
Size:  384 reactions
Description:  The BRG1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRG1 binding to its substrate. The key to the BRG1 Inhibitor Screening Assay Kit is the specific binding of the BRG1 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRG1 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Applications:  Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Kit Components:  The kit comes in a convenient AlphaLISA format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged BRG1 bromodomain to perform a total of 384 enzyme reactions.
Warning:  For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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