| Cat.No.: | EKIT-0491 |
| Product Name: | PRMT5 TR-FRET Assay Kit |
| Size: | 384 reactions |
| Description: | The PRMT5 TR-FRET Assay Kit is designed to measure PRMT5 activity in a homogeneous 384 reaction format. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The key to the PRMT5 TR-FRET Assay Kit is a highly specific antibody that recognizes methylated substrate. |
| Background: | PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. PRMT5 is a critical regulatory-protein linked to genome organization, cell cycle regulation, and stem cell differentiation. PRMT5 transfers methyl groups from S-adenosylmethoinine (SAM) to form mono and symmetric di-methylated arginine residues in histone proteins, transcription factors, and other regulatory proteins. Given its regulatory function, PRMT5 is believed to contribute to tumorigenesis. Its dysregulated activity has been associated with many cancers including lymphomas, lung, and breast cancer. Additionally, PRMT5 has been linked to other diseases including kidney and heart disease as well as neurological disorders such as Huntington’s and Alzheimer’s diseases. |
| Applications: | For studying enzyme kinetics and HTS applications. |
| Kit Components: | The PRMT5 TR-FRET Assay Kit comes in a convenient format, with histone H4 peptide substrate, a Eu-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, FRET detection buffer, dye-labeled acceptor, and purified PRMT5. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| BSM-0047 | SGC707 | Inquiry |
| ◆ Antibodies | ||
| EAb-0048 | PRMT6 Polyclonal Antibody | Inquiry |
| EAb-0049 | PRMT7 Polyclonal Antibody | Inquiry |
| EAb-0050 | PRMT1 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| Pr-SET7 | PRC1 | PRC2 | PRDM1 |
| PRDM10 | PRDM11 | PRDM12 | PRDM13 |
| PRDM14 | PRDM15 | PRDM16 | PRDM17 |
| PRDM2 | PRDM3 | PRDM4 | PRDM5 |
| PRDM6 | PRDM7 | PRDM8 | PRDM9 More > |
| PREP1 | PRIM2 | PRKAA2 | PRKCA |
| PRKCB | PRKCE | PRKDC | PRKRIP1 |
| PRMT | prmt1 | PRMT2 | PRMT3 |
| PRMT5 | prmt6 | PRMT7 | PRMT8 |
| PRMT9 | Progerin | Protamine 2 | Prox1 |
| PRPF31 | PRPF40A | PRSS12 | PRSS55 |
| PRTFDC1 | |||
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