| Cat.No.: | EKIT-0488 |
| Product Name: | PRMT4 Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
| Background: | PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17. |
| Applications: | For studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Kit Components: | Kit comes in a convenient format, with 8-well strips precoated with the specific PRMT4 peptide substrate, reagents, and purified PRMT4 enzyme for 96 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0008 | PRMT4 peptide substrate, Biotin-labeled | Inquiry |
| ◆ Cell Lines | ||
| CL-0050 | Human CARM1 Knockout Cell Line 8bp deletion | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0123 | Ellagic acid | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0134 | Recombinant Human CARM1 293 Cell Lysate | Inquiry |
| EL-0148 | Recombinant Human CAMTA2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| CABIN1 | CAF1 | CAMKIV | CAMTA2 |
| CAPNS2 | CARHSP1 | CARM1 | CASC3 |
| CASP | CASP1 | CASP3 | |
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