Cat.No.: | EKIT-0479 |
Product Name: | SetDB1 Chemiluminescent Assay Kit |
Size: | 96 reactions |
Description: | The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications: | For studying enzyme kinetics and HTS applications. |
Kit Components: | Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase buffer, and purified enzyme |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Bioactive Small Molecules | ||
BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
◆ Antibodies | ||
EAb-0030 | SETD8 Polyclonal Antibody | Inquiry |
EAb-0031 | SETDB1 Polyclonal Antibody | Inquiry |
EAb-0032 | Setd1a Polyclonal Antibody | Inquiry |
EAb-0033 | Setd1b Polyclonal Antibody | Inquiry |
Related Gene / Proteins | |||
SENP3 | SENP8 | SET | SET1 |
SET2 | SET7 | SET8 | SET9 |
SETBP1 | SETD1A | SETD1B | SETD2 |
SETD3 | SETD5 | SETD6 | SETD7 |
SETD8 | SETD9 | SetDB1 | SETDB2 More > |
SETMAR |
USA
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