Cat.No.: | EKIT-0476 |
Product Name: | SMYD3 Homogeneous Assay Kit |
Size: | 384 reactions |
Description: | The SMYD3 Homogeneous Assay Kit is designed to measure SMYD3 activity for screening and profiling applications. SMYD3 is a histone methyltransferase that promotes diand trimethylation of lysine4 of histone H3. It also exhibits methylation activity toward a lysine residue of MAPK/ERK Kinase Kinase 2 (MAP3K2). The key to the SMYD3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing SMYD3 enzyme is incubated with the substrate for three hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. |
Applications: | For studying enzyme kinetics and HTS applications. |
Kit Components: | The SMYD3 Homogeneous Assay Kit comes in a convenient AlphaLISA format, with GST-tagged SMYD3 substrate, primary antibody, methylation assay buffer, and purified SMYD3 for 384 enzyme reactions. |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Antibodies | ||
EAb-0019 | SMARCA4 Polyclonal Antibody | Inquiry |
◆ Extracts & Lysates | ||
EL-0063 | Recombinant Human SMARCC2 293 Cell Lysate | Inquiry |
EL-0064 | Recombinant Human SMARCB1 Cell Lysate | Inquiry |
◆ Bioactive Small Molecules | ||
BSM-0080 | AZ-505 ditrifluoroacetate | Inquiry |
◆ Research Kits | ||
EKIT-0120 | BRG1 bromodomain TR-FRET Assay Kit | Inquiry |
Related Gene / Proteins | |||
SMAD3 | SMAD4 | SMARCA1 | SMARCA2 |
SMARCA4 | SMARCA5 | SMARCA6 | SMARCAD1 |
SMARCAL1 | SMARCB1 | SMARCC1 | SMARCC2 |
SMARCD1 | SMARCD2 | SMARCD3 | SMARCE1 |
SMC1-α | SMC3 | SMC4 | SMCHD1 More > |
SMCX | SMN | SMN1 | SMN2 |
SMOC2 | SMRT | SMT3 | SMUG1 |
SMURF2 | SMYD1 | SMYD2 | SMYD2B |
SMYD3 | SMYD4 | SMYD5 |
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