| Cat.No.: | EKIT-0349 |
| Product Name: | HDAC8 Activity Assay Kit (Fluorometric) |
| Assay Type: | Enzyme activity |
| Description: | HDAC8 Activity Assay Kit (Fluorometric) detects HDAC activity in lysates.Primarily, the HDAC8 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the HDAC8 Activity Assay Kit (Fluorometric) after immunoprecipitation with an appropriate HDAC8 specific antibody.Applications for this kit include:1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8 (class I).2. Screening inhibitors or activators of HDAC8.3. Detecting the effects of pharmacological agents on HDAC8. |
| Background: | Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4), allowing the histones to wrap the DNA more tightly.HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity. Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. |
| Detection Method: | Fluorescent |
| Target Species: | Human |
| Tissue Specificity: | Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0006 | Recombinant Human HDAC1 293 Cell Lysate | Inquiry |
| EL-0007 | Recombinant Human HDAC2 293 Cell Lysate | Inquiry |
| EL-0008 | Recombinant Human HDAC3 Cell Lysate | Inquiry |
| EL-0009 | Recombinant Human HDAC4 Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0007 | Tubacin | Inquiry |
| Related Gene / Proteins | |||
| HDAC | HDAC1 | HDAC10 | HDAC11 |
| HDAC2 | HDAC3 | hdac4 | hdac5 |
| HDAC6 | hdac7 | HDAC8 | hdac9 |
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