| Cat.No.: | EKIT-0285 |
| Product Name: | MLL1 Complex Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The MLL1 Complex Direct Activity Chemiluminescent Assay kit is designed to measure MLL1 activity for screening and profiling applications, using purified MLL1 and its complex components: WDR5, RbBP5,Ash2, and DPY30. The key to the MLL1 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. |
| Kit Components: | MLL1/WDR5/Ash2L/RbBP5/DPY30: 25 µg; -80°C 20 µM S-adenosylmethionine: 250 µl; -80°C Primary antibody 2: 12.5 µl; -80°C 4x HMT assay buffer 1: 3 ml; -20°C Secondary HRP-labeled antibody 1: 10 µl; -80°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate (2 components): 6 ml; each +4°C 96-well plate: 1; plate +4°C |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0170 | MI-3 | Inquiry |
| BSM-0187 | OICR-9429 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0172 | Human KMT2C / MLL3 peptide | Inquiry |
| ◆ Research Kits | ||
| EKIT-0285 | MLL1 Complex Chemiluminescent Assay Kit | Inquiry |
| EKIT-0286 | MLL1 SAM-Screener Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| MLH1 | MLL | mll1 | MLL2 |
| MLL3 | MLL4 | MLL5 | MLLT1 |
| MLLT3 | |||
USA
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