PRMT1 Chemiluminescent Assay Kit

Cat.No.:	EKIT-0283
Product Name:	PRMT1 Chemiluminescent Assay Kit
Size:	96 reactions
Product Overview:	PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Description:	The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Kit Components:	PRMT1 human recombinant enzyme: 10 µg; -80°C 20 µM S-adenosylmethionine: 250 µl; -80°C Primary antibody 4: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C4x HMT assay buffer 2: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
Warning:	For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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