| Cat.No.: | EKIT-0275 |
| Product Name: | HEK 293 RELA Nuclear Translocation Assay Kit |
| Size: | 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well) |
| Product Overview: | Nuclear Translocation cells are engineered to co-two proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA), which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-an untagged secondary protein involved in the pathway of interest. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance. |
| Kit Components: | 1.25 x 10^6 cells per vial in 0.1 mL (kit contains cells, detection reagents and plate(s)) |
| Target Species: | Human |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0113 | Recombinant Human RELA 293 Cell Lysate | Inquiry |
| ◆ Research Kits | ||
| EKIT-0275 | HEK 293 RELA Nuclear Translocation Assay Kit | Inquiry |
| ◆ Cell Lines | ||
| CL-0396 | Human RECQL4 Knockout Cell Line 5bp deletion | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0856 | Recombinant Human RELA, His-tagged | Inquiry |
| PE-0857 | Recombinant Human RELA | Inquiry |
| Related Gene / Proteins | |||
| RECQL4 | rela | Reptin | REST |
| REXO5 | |||
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