| Cat.No.: | EKIT-0274 |
| Product Name: | p53 Transcription Factor Activity Assay Kit |
| Size: | 100 assays |
| Product Overview: | The p53 Transcription Factor Activity Assay Kit is a non-radioactive assay for the quantitative measurement of specific transcription factor DNA binding activity of active p53. A biotin-labeled p53 response element DNA binds specifically to active p53 in nuclear extract and forms a complex. The amount of complex is detected by the p53 specific monoclonal antibody using Streptavidin Plate. A HRP-conjugated secondary antibody provides a sensitive colorimetric readout at 450 nm using TMB as substrate. |
| Description: | The tumor suppressor protein p53 is one of the key players in cancer biology. DNA damage and oxidative stress signals lead to p53 posttranslational modifications and stabilization by binding to its consensus sequence, the p53 response element. Stabilized p53 can interact with other transcriptional regulators for the induction of p53-responsive targets involved in cell cycle progression, cell senescence, or apoptotic cell death. Because the expression and activity of p53 are frequently altered in human cancers, p53 becomes an important drug target as well as a biomarker in carcinogenesis. |
| Kit Components: | Streptavidin Plate: 12 strips x 8 wells; -20°CBinding Buffer: 20 ml; 4°CDTT (1 M): 100 µl; -20°CWash Buffer(10X): 25 ml; 4°CStop Solution: 11 ml; 4°CCapture Oligonucleotide(4 pmole/µl): 100 µl; -20°CCompetitor oligo (50 pmole/µl): 25 µl; -20°CDetection Ab (100X): 100 µl; -20°CHRP Conjugate (100x): 100 µl; -20°CTMB Substrate: 11 ml; -20°Cp53 Positive Control (1 µg/µl): 20 µl; -80°CPlate Sealer: 2; RT |
| Features & Benefits: | • Easy, convenient and time-saving method to assay for human p53• The minimum detectable dose of p53 is typically less than 2 µg per sample• It only recognizes human p53 Transcription Factor |
| Detection Method: | Absorbance (450 nm) |
| Sample Types: | Serum & plasma, Cell culture supernatants, Urine |
| Target Species: | Human |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0182 | NSC 146109 hydrochloride | Inquiry |
| BSM-0404 | Tenovin-3 | Inquiry |
| ◆ Research Kits | ||
| EKIT-0273 | p53 Translocation Assay Kit (Cell-Based) | Inquiry |
| EKIT-0274 | p53 Transcription Factor Activity Assay Kit | Inquiry |
| EKIT-0420 | Human NFkB p50 Transcription Factor Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| p50 | p53 | ||
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