| Cat.No.: | EKIT-0229 |
| Product Name: | DNMT3B Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
| Kit Components: | DNMT3B/3L: 2 x 20 µg; -80°C400 µM S-adenosylmethionine: 2 x 250 µl; -80°C Anti-5-methylcytosine antibody: 25 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x DNMT assay buffer 2: 5 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Plate pre-coated with DNMT substrate: 1; +4°C |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0008 | Vinorelbine Tartrate | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0027 | Recombinant Human DNMT3A 293 Cell Lysate | Inquiry |
| EL-0028 | Recombinant Human DNMT3B 293 Cell Lysate | Inquiry |
| EL-0029 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| EL-0030 | Recombinant Human DNMT3L 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| DNA alkyltransferase | DNAJC2 | DNAS1L1 | DNASE1L3 |
| DNMT | DNMT1 | DNMT2 | DNMT3A |
| DNMT3B | DNMT3L | DNMT4 | |
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