| Cat.No.: | EKIT-0198 |
| Product Name: | Mammalian Chromatin Extraction Kit |
| Product Overview: | Mammalian Chromatin Extraction Kit is used for isolating chromatin or DNA-protein complex from mammalian cells or tissues. |
| Description: | Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, the ChIP method with specific antibodies directly against various transcriptional factors is widely demanded. For performing ChIP, chromatin or DNA-protein complex in cells or tissues should be first isolated. |
| Applications: | The Mammalian Chromatin Extraction Kit is suitable for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays. |
| Kit Components: | 10X Lysis Buffer 11 mlExtraction Buffer 11 mlChromatin Buffer 11 mlProtease Inhibitor Cocktails (1000X)* 110 µl* Spin the solution down to the bottom prior to use. |
| Features & Benefits: | Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 60 minutes.Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc. |
| Sample Types: | Cell, Tissue |
| Target Species: | Mammals |
| Storage: | Upon receipt: (1) Protease Inhibitor cocktails at 4°C; (2) Store remaining components at room temperature.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: Check if any buffers contain salt precipitates before use. If so, shake the buffer until the salts are re-dissolve. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
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