| Cat.No.: | EKIT-0171 |
| Product Name: | Sirtuin Activity Fluorometric Assay Kit |
| Size: | 100 assays |
| Product Overview: | Purified recombinant protein Cell and tissue lysateNuclear ExtractMitochondriaImmunoprecipitated samples |
| Description: | Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC's also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC's in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 µU of recombinant human SIRT6. |
| Applications: | Detection of Sirtuin Activity in variety of samples |
| Kit Components: | • Sirtuin Assay Buffer • Homogenization Buffer • 1M DTT • Substrate (in DMSO) • NAD • Positive Control • Trichostatin A (in DMSO) • Developer • AFC Standard (in DMSO) (1 mM) |
| Detection Method: | Fluorescence (Ex/Em = 400/505 nm) |
| Sample Types: | Purified recombinant protein, Cell and tissue lysate, Nuclear Extract, Mitochondria, Immunoprecipitated samples |
| Storage: | -20°C |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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