| Cat.No.: | EKIT-0168 |
| Product Name: | Methyltransferase HT activity Kit |
| Size: | 96 wells |
| Product Overview: | Universal-suitable for any SAM-dependent MTFlexible-add desired amount of SAMSensitive-fluorescent detection allows for both DNA and protein assaysSimple-homogeneous mix-and-read plate-based assay |
| Description: | The Methyltransferase HT activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. This kit is ideal for high throughout (HT) applications as the SAM is provided separately giving you the flexibility to titrate the SAM to be in excess for your specific MT of interest. This is ideal for driving kinetics of low turnover enzymes and getting more utility by using less precious sample. |
| Applications: | Fluorescence microscopy, HTS. For the measurement of AdoMet-dependent methyltransferase activity in purified in vitro samples that do not contain reductants from any species. |
| Kit Components: | Black Microtiter plate, SAM-Free Reaction buffer, Assay buffer, Detection solution, SAM, Positive control |
| Storage: | Store assay buffer +4°C, and all other components at -20°C. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
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