| Cat.No.: | PE-3835 |
| Product Name: | SIRT5 (human), (recombinant) (His-tag) |
| Description: | This recombinant preparation, expressed in E. coli, comprises residues 37-310 of SIRT5 isoform 1, with an N-terminal His-tag. |
| Background: | SIRT5, along with SIRTs 3 and 4, is one of three human mitochondrial sirtuins (homologs of yeast Sir2). SIRT5, which is localized to both the mitochondrial matrix and the intermembrane space, is an NAD+-dependent lysine deacetylase (class III HDAC). SIRT5's only known physiological target is the intermembrane space protein, cytochrome c, suggesting the possibility of regulatory roles in metabolism and/or apoptosis. SIRT5 has also been shown to deacetylate and thereby upregulate the matrix urea cycle enzyme carbamoyl phosphate synthetase 1. This implies an important role for SIRT5 in ammonia detoxification and adaptation to elevated amino acid catabolism during starvation, calorie restriction or a high protein diet. SIRT5 is most strongly expressed in thymus, lymphoblasts and heart muscle cells and its chromosomal location (6p23) has been implicated in chromosomal abnormalities associated with malignancies. |
| Applications: | Useful for studies of enzyme kinetics, modulator activity, drug discovery. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Molecular Weight: | ~32.3kDa |
| Purity: | ≥95% (SDS-PAGE) |
| Formulation: | 25mM TRIS, pH 7.5, 100mM sodium chloride, 5 mM DTT, containing 10% (v/v) glycerol. |
| Accession#: | Q9NXA8 |
| Alternative Names: | Sirtuin 5, Sir2-like protein 5, SIR2L5, NAD-dependent deacetylase Sirtuin-5 |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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