| Cat.No.: | PE-3787 |
| Product Name: | PCAF, mouse recombinant |
| Background: | The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. p300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Molecular Weight: | ~92 kDa (SDS-PAGE). Mouse PCAF is fused to a His-tag. |
| Purity: | Purity by SDS-PAGE ≥95% |
| Formulation: | 50 mM Tris-HCl, pH 7.5, containing 100 mM sodium chloride, 50 mM imidazole, 0.2% NP-40 and 10% glycerol. |
| Accession#: | Q9JHD1 |
| Alternative Names: | Histone Acetyltransferase PCAF; Histone Acetyltransferase KAT2B; Lysine Acetyltransferase 2B; P/CAF; P300/CBP-associated Factor. |
| Expression System: | Sf21 Insect Cells |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0039 | Embelin | Inquiry |
| BSM-0073 | Anacardic Acid | Inquiry |
| ◆ Antibodies | ||
| EAb-0060 | PCAF Polyclonal Antibody | Inquiry |
| EAb-0073 | KAT8 Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0083 | Human KAT2A Knockout Cell Line 1bp insertion | Inquiry |
| Related Gene / Proteins | |||
| Kaiso | KANSL2 | KAP1 | KAT13A |
| KAT13D | KAT2A | KAT2B | KAT4 |
| KAT5 | KAT6A | KAT6B | KAT7 |
| KAT8 | KAT9 | PCAF | PCBP2 |
| PCBP3 | PCBP4 | PCGF2 | PCGF6 More > |
| PCL2 | PCNA | ||
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