| Cat.No.: | PE-3710 |
| Product Name: | Recombinant EZH1 complex |
| Product Overview: | Recombinant EZH1 complex contains full length human EZH1 protein (Accession number NP_001982.2) with N-terminal FLAG-Tag and MW = 86.3 kDa; full length human SUZ12 (Accession No. NP_056170.2), with N-terminal 6×His-Tag and MW = 86.1 kDa; full length human EED (Accession No. NP_003788.2), N-terminal 6×His-Tag and MW = 53.2 kDa; full length human RbAp46 (Accession No. NP_001185648.1), N-terminal 6×His-Tag and MW = 55.3 kDa, and full length human RbAp48 (Accession No. NP_005601.1), N-terminal 6×His-Tag and MW = 50.7 kDa, all individually expressed in Sf9 cells. The recombinant protein is >95% pure by SDS-PAGE. |
| Background: | EZH1 (Enhancer Of Zeste Homolog 1 ) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates ‘Lys-27’ of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate ‘Lys-27’ of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| Tag: | FLAG & His |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0006 | GSK343 | Inquiry |
| BSM-0059 | 3-Deazaneplanocin A | Inquiry |
| BSM-0060 | 3-Deazaneplanocin A hydrochloride | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0024 | Recombinant Human EZH1 293 Cell Lysate | Inquiry |
| EL-0025 | Recombinant Human EZH2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ezh1 | EZH2 | ||
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
