| Cat.No.: | PE-3707 |
| Product Name: | Recombinant KMT2B (MLL4) complex |
| Product Overview: | Recombinant KMT2B (MLL4) complex contains amino acids 2551 - 2715 of the human MLL4 protein (accession number NP_055542.1) with N-terminal GST-Tag and MW = 47.6 kDa; full length human WDR5 (GenBank Accession No.NM_017588), with N-terminal 6×His-Tag and MW = 35 kDa; full length human ASH2L (GenBank Accession No. NM_001105214), N-terminal 6×His-Tag and MW = 61 kDa; full length human RbBP5 (GenBank Accession No. NM_005057), N-terminal 6×His-Tag and MW = 60 kDa, and full length human DPY30 (GenBank Accession No. NM_032574), N-terminal 6×His-Tag and MW =12 kDa, all individually expressed in E. coli cells. The recombinant protein is >85% pure by SDS-PAGE. |
| Background: | KMT2B (MLL4) (Myeloid/lymphoid or mixed-lineage leukemia 4) is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL4) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in β-globin locus transcription regulation by being recruited by Nfe2. KMT2B (MLL4) is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100. |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0170 | MI-3 | Inquiry |
| BSM-0187 | OICR-9429 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0172 | Human KMT2C / MLL3 peptide | Inquiry |
| ◆ Cell Lines | ||
| CL-0216 | Human KMT2A Knockout Cell Line 32bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0285 | MLL1 Complex Chemiluminescent Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| KMT1B | KMT1E | KMT2A | KMT2B |
| KMT2C | KMT2D | KMT2E | KMT3A |
| KMT3B | KMT3C | KMT5A | KMT6 |
| MLH1 | MLL | mll1 | MLL2 |
| MLL3 | MLL4 | MLL5 | MLLT1 More > |
| MLLT3 | |||
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