| Cat.No.: | PE-3706 |
| Product Name: | Recombinant KMT2C (MLL3) complex |
| Product Overview: | Recombinant KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30) was generated using a peptide corresponding to amino acids 4740-4911 (accession number NP_733751.2) of the KMT2C protein that was expressed in E. coli. Recombinant KMT2C (MLL3) complex contains an N-terminal GST-Tag with a molecular weight of 46 kDa; human WDR5 (accession number NM_017588) that contains an N-terminal His-Tag with a molecular weight of 35 kDa; human ASH2L (accession number NM_001105214) that contains an N-terminal His-Tag with a molecular weight of 61 kDa; human RBBP5 (accession number NM_005057) that contains an N-terminal His-Tag with a molecular weight of 60kDa; and human DPY30 (accession number NM_032574) that contains an N-terminal His-Tag with a molecular weight of 12 kDa. All Recombinant MLL3/WDR5/ASH2L/RBBP5/DPY30 complex proteins were individually expressed in an E. coli expression system. The recombinant protein is >90% pure by SDS-PAGE. |
| Background: | The KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The KMT2C (also known as MLL3) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0170 | MI-3 | Inquiry |
| BSM-0187 | OICR-9429 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0172 | Human KMT2C / MLL3 peptide | Inquiry |
| ◆ Cell Lines | ||
| CL-0216 | Human KMT2A Knockout Cell Line 32bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0285 | MLL1 Complex Chemiluminescent Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| KMT1B | KMT1E | KMT2A | KMT2B |
| KMT2C | KMT2D | KMT2E | KMT3A |
| KMT3B | KMT3C | KMT5A | KMT6 |
| MLH1 | MLL | mll1 | MLL2 |
| MLL3 | MLL4 | MLL5 | MLLT1 More > |
| MLLT3 | |||
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