| Cat.No.: | PE-3702 |
| Product Name: | Recombinant NSD1-SET protein |
| Product Overview: | Recombinant NSD1-SET peptide that includes amino acids 1852-2082 that contains the SET domain sequence of the human NSD1 protein (accession number NP_071900.2) was expressed in E. coli cells and contains an N-terminal GST-Tag with an observed molecular weight of 51 kDa. The recombinant protein is >95% pure by SDS-PAGE. |
| Background: | NSD1 (Nuclear Receptor Binding SET Domain Protein 1) is a histone methyltransferase that preferentially methylates lysine 36 of histone H3 and lysine 20 of histone H4 in vitro. NSD1 is a transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 protein contains a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor-associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor as well as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su(var), Enhancer of zeste (EZH2), and Trithorax Domain Protein 1 on chromosome 5 and Nucleoporin, 98 kd on chromosome 11.NSD1-SET includes the SET domain of NSD1. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris, pH 7.4, 150 mM NaCl and 5% glycerol. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| ◆ Antibodies | ||
| EAb-0030 | SETD8 Polyclonal Antibody | Inquiry |
| EAb-0031 | SETDB1 Polyclonal Antibody | Inquiry |
| EAb-0032 | Setd1a Polyclonal Antibody | Inquiry |
| EAb-0033 | Setd1b Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| nsd1 | NSD2 | NSD3 | NSMCE2 |
| NSUN1 | NSUN2 | NSUN3 | NSUN4 |
| NSUN5 | NSUN6 | NSUN7 | SENP3 |
| SENP8 | SET | SET1 | SET2 |
| SET7 | SET8 | SET9 | SETBP1 More > |
| SETD1A | SETD1B | SETD2 | SETD3 |
| SETD5 | SETD6 | SETD7 | SETD8 |
| SETD9 | SetDB1 | SETDB2 | SETMAR |
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