Recombinant SETD8 protein

Cat.No.:	PE-3677
Product Name:	Recombinant SETD8 protein
Product Overview:	Full length recombinant SETD8 protein (accession number NP_065115.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 42.2 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Background:	SETD8 (SET Domain Containing (Lysine Methyltransferase) 8) is Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.It specifically catalyzes monomethylation of histone H4 lysine 20 (H4K20) which is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. H4K20me0 is also involved in DNA damage response. Besides H4K20, SETD8 monomethylates non-histone substrates including proliferating cell nuclear antigen (PCNA) and promotes carcinogenesis by deregulating PCNA expression. It also mediates monomethylation of p53/TP53 at 'Lys-382'and leads to repress p53/TP53-target genes.
Applications:	Suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Storage:	Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
Appearance:	Liquid
Species:	Human
Formulation:	25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100 and 0.2 mg/ml 3× Flag peptide.
Tag:	FLAG
Expression System:	Baculovirus
Warning:	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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