| Cat.No.: | PE-3631 |
| Product Name: | Recombinant KAT2B / PCAF (715-829) protein |
| Product Overview: | The peptide corresponding to amino acids 715 - 829 that contains the bromodomain sequences of KAT2B / PCAF (accession number NM_003884.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.8 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K36, H4K8, H4K16, H4K20 and TATK50. The recombinant protein is >90% pure by SDS-PAGE. |
| Background: | P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. KAT2B / PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. KAT2B / PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. KAT2B / PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, KAT2B / PCAF is recruited by the viral protein TAT to regulate TAT’s transactivating activity and may help induce chromatin remodeling of proviral genes. |
| Applications: | Suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. |
| Tag: | His & FLAG |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0039 | Embelin | Inquiry |
| BSM-0073 | Anacardic Acid | Inquiry |
| ◆ Antibodies | ||
| EAb-0060 | PCAF Polyclonal Antibody | Inquiry |
| EAb-0073 | KAT8 Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0083 | Human KAT2A Knockout Cell Line 1bp insertion | Inquiry |
| Related Gene / Proteins | |||
| Kaiso | KANSL2 | KAP1 | KAT13A |
| KAT13D | KAT2A | KAT2B | KAT4 |
| KAT5 | KAT6A | KAT6B | KAT7 |
| KAT8 | KAT9 | PCAF | PCBP2 |
| PCBP3 | PCBP4 | PCGF2 | PCGF6 More > |
| PCL2 | PCNA | ||
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