| Cat.No.: | PE-3618 |
| Product Name: | Recombinant TAF1 (1522-1656) protein |
| Product Overview: | The protein corresponding to amino acids 1522-1656 that contains the bromodomain sequence of TAF1 (accession number NP_004597.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 19.3 kDa. It shows binding specificity for acetylated H3K14. |
| Background: | TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle. |
| Applications: | Suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. |
| Tag: | His & FLAG |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0017 | TAF1 Polyclonal Antibody | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0056 | Recombinant Human TAL1 293 Cell Lysate | Inquiry |
| EL-0108 | Recombinant Human TAF7 293 Cell Lysate | Inquiry |
| EL-0178 | Recombinant Human TADA1 Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0121 | Human TAP2 Knockout Cell Line | Inquiry |
| Related Gene / Proteins | |||
| TADA1 | TADA1L | TADA2A | TADA2B |
| TADA3 | TAE1 | TAF-I | taf1 |
| TAF1L | TAF6L | TAF7 | TAF7L |
| TAL1 | TAP2 | TAZ | |
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