| Cat.No.: | PE-3607 |
| Product Name: | Recombinant Histone H2A.Z/H2B dimer |
| Product Overview: | Recombinant histone H2A.Z/H2B Dimer consists of full length human histones H2A.Z (accession number NP_002097.1) and full length human histone H2B (accession number NP_003509.1). Histone H2A.Z and H2B were expressed in E. coli cells. Recombinant Histone H2A.Z/H2B Dimer was assembled by dialysis and purified by gel filtration. The molecular weight of histone H2A.Z/H2B Dimer is 27.2 kDa. |
| Background: | Histone H2A and H2B are the core components of the nucleosome. H2A and H2B assemble H2A/H2B dimer, and two H2A/H2B dimers combine with H3/K4 tetramer to form histone octamer. The histone octamer is wrapped around by 146 bp DNA to assemble a nucleosome, which is the basic structural unit of chromatin in vivo.H2A.Z (also known as H2AFZ, Histone Family Member Z) is a histone H2A family member. It is highly conserved fromyeast to human, with 90% of its primary sequence preserved among different species, showing only 60% homology with canonical histone H2A. H2A.Z is found in approximately 10% of mammalian nucleosomes. H2A.Z has been one of the most studied H2A variants in recent years. It plays an important role in different biological processes such as DNA replication, DNA repair, transcription regulation, cell cycle, spermatogenesis, chromosome segregation, centromere structure and maintenance of heterochromatic/euchromatic status. However, different studies reported diverse conclusions in the nucleosome stability and transcriptional regulation. The contradictory roles of H2A.Z in vivo might be explained by different combinations of H2A.Z with other epigenetic regulators, PTMs on H2A.Z and interaction withchromatin binding proteins. |
| Applications: | Suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 10 mM Tris-HClpH 7.4, 2 M NaCl, 1 mM EDTA, and 5 mM b-mercaptoethanol. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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