| Cat.No.: | PE-3457 |
| Product Name: | Recombinant METTL1 / WDR4 complex |
| Product Overview: | Recombinant METTL1 / WDR4 Complex that includes full length human WDR4 protein (accession number NP_061139.2) and full length human METTL1 protein (accession number NP_005362.3) with an N-terminal FLAG-Tag was expressed in a baculovirus expression system. The molecular weights of WDR4 and METTL1 are 45.5 kDa and 32.7 kDa, respectively. |
| Background: | WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his andtrp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. METTL1 (Methyltransferase-Like Protein 1), also known as TRM8, contains a conserved S-adenosylmethionine-binding motif and is inactivated by the protein kinase B (PKB) and ribosomal S6 kinase (RSK) under growth factor stimulation. WRD4 and METTL1 that form a complex are required for tRNA (guanine(46)-N(7))-methyltransferase activity. More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modification occurs at position 46 in the variable region and is a product of tRNA (m7G46) methyltransferase. The m7G46 modification forms a tertiary base pair with C13-G22, and stabilizes the tRNA structure. |
| Applications: | Suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0005 | WDR5 Polyclonal Antibody | Inquiry |
| EAb-0067 | MeCP2 Polyclonal Antibody | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0043 | Recombinant Human MECP2 293 Cell Lysate | Inquiry |
| EL-0052 | Recombinant Human WDTC1 293 Cell Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0109 | Human MECP2 Knockout Cell Line 1bp insertion | Inquiry |
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