| Cat.No.: | PE-3426 |
| Product Name: | Recombinant MLLT3 / AF9 (1-138) protein |
| Product Overview: | Recombinant MLLT3 / AF9 (1-138) protein was expressed in E.coli as the full length protein (accession number NP_004520.2) with an N-terminal 6xHis Tag. The molecular weight of the protein is 19.8 kDa. |
| Background: | MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein), also known as YEATS3 or AF9, is a chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. MLLT3 recognizes and binds histone H3 crotonylated at Lys-9 (H3K9cr), and with slightly lower affinity histone H3 crotonylated at Lys-18 (H3K18cr). It also recognizes and binds histone H3 acetylated at Lys-9 (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. |
| Applications: | Suitable for use in binding assay, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris-HCl pH 7.4, 500 mM NaCl, 2 mM EDTA, 2 mM DTT, 10% glycerol, 0.5 mM TCEP. |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0170 | MI-3 | Inquiry |
| BSM-0187 | OICR-9429 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0172 | Human KMT2C / MLL3 peptide | Inquiry |
| ◆ Research Kits | ||
| EKIT-0285 | MLL1 Complex Chemiluminescent Assay Kit | Inquiry |
| EKIT-0286 | MLL1 SAM-Screener Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| MLH1 | MLL | mll1 | MLL2 |
| MLL3 | MLL4 | MLL5 | MLLT1 |
| MLLT3 | |||
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