| Cat.No.: | PE-3421 |
| Product Name: | Recombinant NFκB p65 protein |
| Product Overview: | Recombinant NFκB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration. |
| Background: | NFκB p65 is a subunit of the NFκB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. NFκB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. |
| Applications: | Suitable for use in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10% glycerol) and 1 M DTT. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0201 | Recombinant Human NFKBIL2 293 Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0294 | Wedelolactone | Inquiry |
| ◆ Research Kits | ||
| EKIT-0429 | NFkB p65 Transcription Factor Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-1897 | NFκB p65 phospho Ser536 Polyclonal Antibody | Inquiry |
| EAb-1898 | NFκB p65 phospho Ser529 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| NF-1A | NF-1B2 | NFATC2 | NFKBIL2 |
| NFκB | NFκB1 | NFκB3 | p65 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
