| Cat.No.: | PE-3420 |
| Product Name: | Recombinant NFκB1 p50 (1-434) protein |
| Product Overview: | Recombinant NFKB1 / p50 (1-434) protein including amino acid 1 - 434 of NFKB1 p105 subunit (accession number NP_003989.2) was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of NFKB1 / p50 (1-434) is 73.9 kDa. |
| Background: | NFKB1 (Nuclear Factor Kappa B Subunit 1, also known as CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFκB, p105, p50) is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. The precursor NFKB1 is a 105 kDa protein (p105). The 105 kDa protein can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein (p50). The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB complex plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. |
| Applications: | Suitable for use in protein-protein interaction, in vitro transcription assay, binding assay. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0182 | NSC 146109 hydrochloride | Inquiry |
| BSM-0294 | Wedelolactone | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0201 | Recombinant Human NFKBIL2 293 Cell Lysate | Inquiry |
| ◆ Research Kits | ||
| EKIT-0273 | p53 Translocation Assay Kit (Cell-Based) | Inquiry |
| EKIT-0274 | p53 Transcription Factor Activity Assay Kit | Inquiry |
| Related Gene / Proteins | |||
| NF-1A | NF-1B2 | NFATC2 | NFKBIL2 |
| NFκB | NFκB1 | NFκB3 | p50 |
| p53 | |||
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