| Cat.No.: | PE-3419 |
| Product Name: | Recombinant NFκB3 (RELA / p65) protein |
| Product Overview: | Recombinant human NFκB3 (RELA / p65) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_068810.3) with an N-terminal FLAG tag. The molecular weight of the protein is 61.3 kDa. |
| Background: | NFκB3 (Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3), also known as RELA (V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A) or p65, is a ubiquitous transcription factor involved in several biological processes. NFκB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.The NFκB dimers bind at κ-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different κ-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NFκB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NFκB complexes are held in the cytoplasm in an inactive state complexed with members of the NFκB inhibitor (Iκ-B) family. In a conventional activation pathway, Iκ-B is phosphorylated by Iκ-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NFκB complex which translocates to the nucleus. NFκB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NFκ-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of Iκ-B upon NFκB in the cytoplasm is exerted primarily through the interaction with p65. |
| Applications: | Suitable for use in protein-protein interaction, in vitro transcription assay, binding assay. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0113 | Recombinant Human RELA 293 Cell Lysate | Inquiry |
| EL-0201 | Recombinant Human NFKBIL2 293 Cell Lysate | Inquiry |
| ◆ Research Kits | ||
| EKIT-0275 | HEK 293 RELA Nuclear Translocation Assay Kit | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0294 | Wedelolactone | Inquiry |
| ◆ Cell Lines | ||
| CL-0396 | Human RECQL4 Knockout Cell Line 5bp deletion | Inquiry |
| Related Gene / Proteins | |||
| NF-1A | NF-1B2 | NFATC2 | NFKBIL2 |
| NFκB | NFκB1 | NFκB3 | p65 |
| RECQL4 | rela | Reptin | REST |
| REXO5 | |||
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